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Fig. 3. Migrating neural crest cells forming chains through the rostral sclerotome, extending from the DRG to the dorsal aorta. (A) Lateral view of sagittal explant of pMES fluorescently labeled neural crest cells overlaid on brightfield image. (B) Fluorescence of labeled neural crest cells. (C-F) Single chain extending from neural tube to dorsal aorta. (C) GFP-labeled neural crest cells. (D) Embossed image of chain. (E) Fluorescence and embossed overlay. (F) Individual cells within chain colored separately to define cell boundaries. (G-J) Lateral view of sagittal explant of eGFP fluorescently labeled neural crest cells. (G) Fluorescence of single chain. (H) Embossed image of chain. (I) Individual cells colored separately and overlaid on embossed image. (J) Depth coding of entire chain. Arrow indicates dorsal (D) and ventral (V) directions. Scale bars: 50 µm in A,B; 20 µm in C-F; 20 µm in G-J. nt, neural tube; sg, sympathetic ganglia.





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