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Fig. 7. Time-lapse analysis of SG formation in chick trunk sagittal explant imaged medially. Fluorescently labeled neural crest cells in a sagittal explant mounted at embryonic day 3.5. (A) Medial image of neural crest cells dispersed along dorsal aorta forming the SG anlagen. Unbroken lines indicate somite borders; double-ended arrow indicates dorsal (D) and ventral (V) directions. The behavior of cells in the interganglionic space (arrow) between two adjacent SG is illustrated in B-G and B'-G'. (B-G) Segregation of neural crest cells dispersed adjacent to the dorsal aorta into discrete SG anlagen. B'-G' are embossed images of B-G. Broken lines indicate somite borders. All time is in hours. Scale bar: 50 µm.





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