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Fig. 7. Mammary lesions in multiparous K5-{Delta}N57-ßcat mice. (A-C) Squamous metaplasia in a 12-month-old K5-{Delta}N57-ßcat mouse mammary gland. Double immunolabeling with anti-K5 and anti-K10 antibodies (A), anti-K5 and anti hair keratin (B), or immunostaining with anti-ß-catenin antibody (C). ß-catenin-positive nuclei are present (C, arrows). (D) Section through an invasive mammary carcinoma from a 12-month-old K5-{Delta}N57-ßcat mouse stained with anti-K5 and anti-K8 antibodies. Double indirect immunofluorescence. (E) Section through the same tumor stained with anti-ß-catenin and anti-cyclin D1 antibodies. Numerous nuclei stain positive with both antibodies. Scale bars: 50 µm in A,B,D,E; 30 µm in C.





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