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Fig. 8. Levels of Myc, cyclin D1, Timp3 and p63 transcripts in the mammary
hyperplasia in K5-
N57-ßcat mice. (A) Real-time RT-PCR analysis of
levels of Myc, cyclin D1 and Timp3 transcripts in hyperplastic and tumor-free
tissue samples from 12- to 18-month-old transgenic glands (K5-Nß).
Bars represent the mean values x10-2±s.e.m. obtained
in two independent experiments performed in duplicate. (B) Real-time RT-PCR
analysis of TAp63 and Np63 transcript levels in wild-type and
transgenic glands. Bars represent the mean values
x10-4±s.e.m. obtained for RNA samples isolated from
hyperplastic and tumor-free areas from four 12- to 18-month-old
K5-N57-ßcat (K5-Nß) and four wild-type (WT) mice. The
differences in Np63 transcript levels were statistically significant
between wild-type and transgenic tumor-free specimens, wild-type and
transgenic hyperplastic tissue, and transgenic tumor-free and transgenic
hyperplastic tissue (P<0.003, 0.001 and 0.002, respectively). The
differences in TAp63 transcript levels were statistically significant between
wild-type and transgenic tumor-free specimens, and between wild-type and
transgenic hyperplastic tissue (P<0.0015 and 0.002, respectively).
Transcript levels were normalized to the Gapd mRNA.
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