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Fig. 2. GL3 and EGL3 RNA accumulation in the developing root. Whole-mount in situ RNA hybridization of GL3 and EGL3 mRNA in 4-day-old root tips. (A) Hybridization of root tips with GL3/EGL3 sense and antisense RNA probe in wild-type (WT) seedlings. GL3/EGL3 RNAs accumulate preferentially in cells in the H position. (The staining occurs in cells with the higher cell division rate.) The panel on the far right shows that this expression pattern is maintained in epidermal clones. The corresponding sense probe does not show any signal in the root tip. (B) Hybridization of root tips with GL3/EGL3 antisense RNA probe in the wild type, gl3-2, egl3-1 and gl3-2 egl3-1 lines. This indicates that both GL3 and EGL3 RNAs accumulate in the same (H-cell) pattern.





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