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Fig. 7. Localization of a GL3-YFP translation fusion in the root epidermis.
Expression of a GL3::GL3-YFP construct in the developing root
epidermis of 4-day-old gl3-2 mutant seedlings. First three panels:
wide-field fluorescence microscope images. Stars indicate cells files composed
of developing non-hair cells, as determined by tracing the files to the mature
region of the root. Right-most panel: confocal microscope image (cell walls
were counterstained with propidium iodide (red signal) to enhance
visualization). The GL3 fusion protein accumulates predominantly in the nuclei
of the developing non-hair cells, as apparent from their lower cell division
rate and paired nature of these cell files. Consistent with the
GL3::GUS expression pattern (Fig.
1), the GL3 fusion protein also accumulates in the quiescent
center cells of the root meristem.
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