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Fig. 3. Expression of Sema3a in 1- (PN1) and 4-day postnatal (PN4) first
(I), second (II) and third (III) lower molar tooth germs. Bright-(A1,C1) and
dark-field (A2,C2) images of sagittal sections as well as (B)
three-dimensional reconstruction of Sema3a (in red) expression in PN1 first
molar tooth germ shown from below. Sema3a is absent from the sites of
the secondary anterior (mesial) and posterior (distal) secondary apical
foramina. The inner dental epithelium (gray) is 30% transparent to better
visualize Sema3a expression in the dental papilla around the secondary apical
foramina. Three-dimensional reconstruction was rendered using a perspective
camera view. Scale bars: 200 µm. White arrows indicate Sema3a
expression in dark-field images. a, developing alveolar bone; cl, cervical
loop; d, dentin; e, enamel; dp, dental papilla; ode, outer dental epithelium;
p, developing pulp floor; sr, stellate reticulum.
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