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Fig. 6. Effects of mutation of the DIV gene on development of the ventral
petal of A. majus and expression of AmMYBML1. (A) Light
micrograph of the corolla tube of the ventral petal of a wild-type flower of
A. majus, showing the mass of hairs in the throat of the tube that
collect pollen, the strips of yellow hairs (nectar guides) and the folds of
the ventral petal that reinforce the hinge. (B) Light micrograph of the
corolla tube of the ventral region of a div mutant flower for
comparison with A. The div mutant petal lacks the hairs in the throat
of the tube and the folds around the hinge. (C) SEM of the corolla tube of the
ventral petal of a wild-type flower. Scale bar: 1mm. (D) SEM of the corolla
tube of the ventral region of the corolla of a div mutant flower.
Scale bar: 1 mm. (E) RNA gel blots showing the effects of the div
mutation on expression of AmMYBML1 and MIXTA. Div and
div represent RNA from wild-type corollas and div mutant
corollas, respectively. (F) SEM of folds of ventral petal in the region of the
hinge of a wild-type flower. Scale bar: 100 µm. (G) SEM of region
equivalent to F around the hinge in div mutant flower. Only very
slight undulations of tissue are observed compared with F. Scale bar: 100
µm. (H) SEM of surface of ventral petal lobe of a div mutant
flower. The hinge is on the left. F indicates flat cells; C indicates conical
cells. Scale bar: 1 mm. (I) SEM of petal epidermal cells of ventral petal lobe
in a region more distal to the hinge (than in G) in a wild-type flower. Scale
bar: 100 µm. (J) SEM of petal epidermal cells of petal lobe in the ventral
position in a region more distal to the hinge (than in H) in a div
mutant flower. Scale bar: 100 µm.
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