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Fig. 7. Effects of weak alleles of the DEF gene on development of the
ventral petal of A. majus and expression of AmMYBML1. (A)
Light micrograph of the corolla tube of the ventral region of a
def101 mutant flower for comparison with wild type shown in
Fig. 6A. The def101
mutant petal has many fewer hairs in the throat of the corolla tube and less
folds around the hinge. (B) SEM of the corolla tube of the ventral petal of a
def101 mutant flower. There are far fewer hairs in the throat of the
tube and fewer folds around the hinge. Scale bar: 1 mm. (C) SEM of folds of
ventral petal in the region of the hinge of a def101 mutant ventral
petal for comparison to the structure of this region in the wild-type corolla
shown in Fig. 6F. (D) SEM of
surface of the ventral petal lobe of a def101 mutant flower in the
region of the hinge. The hinge is on the left. All the epidermal cells are
flat. This should be compared with the wild type shown in
Fig. 6F. (E) Quantitative
RT-PCR of AmMYBML1 transcript levels in corollas of wild type
(Def), def101 mutant, def chlor mutant
and lines heterozygous for def101 and def chlor.
Transcript levels of AmMYBML1 should be compared with the levels of
control gene, encoding ubiquitin (UBI) in the same tissues.
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