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Fig. 4. prdm1 misexpression phenotype. (A-D) Lateral view, anterior to the
left. (E,F) Dorsal view, anterior to the top. (G,H,M,N,S,T) Lateral view,
dorsal to the right. (I-L,O,P) Animal view, dorsal to the right. (Q,R) Animal
view, dorsal to the bottom. (S,T) Dorsal view, animal to the top.
(A,E,G,I,K,M,O,Q,S) Untreated controls (ctrl). (B-D,F,H,J,L,N,P,R,T) Embryos
microinjected with synthetic prdm1 RNA (Prdm1). (A-D) Overall
morphology at 1 dpf. (E,F) six3b, pax2a, krox20 expression in the
developing brain at early somitogenesis; somitic dlc expression for
staging purposes. (G,H) boz; (I,J) gsc; (K,L) chd;
(M,N) szl; and (O,P) bmp4 expression. (Q,R) hgg1
and dlx3b expression at the start of somitogenesis. (S) six3b,
pax2a, krox20 expression in untreated and (T) prdm1-injected
boz embryos with low phenotypic penetrance and expressivity. (U)
prdm1-misexpression causes increased penetrance and expressivity of
the boz phenotype monitored by reduction of six3b
expression; independent females (#1-3). For details see text. Scale bars: 200
µm. d, dorsal; pp, prechordal plate. Animal pole highlighted by a star.
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