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Fig. 5. Effects of prdm1 misexpression on mesendoderm formation. (A-F) Animal view, dorsal to the right. (G,H) Dorsal view, animal to the top. (A,C,E,G) Untreated controls (ctrl). (B,D,F,H) Embryos microinjected with 100 pg of synthetic prdm1 mRNA (Prdm1). (A,B) ntl expression in mesoderm. (C,D) wnt8a expression in mesoderm. (E,F) mix expression in endoderm. (G,H) sox17 expression in endoderm and dorsal forerunner cells. Scale bar: 200 µm (B). df, dorsal forerunner cells; en, endoderm.





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