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Fig. 7. Expression of marker genes of early forebrain development in E10.5 embryos.
(A,B) In-situ hybridization (ISH) of frontal head aspects (upper panel) and
coronal forebrain sections (lower panel) demonstrate reduced signals for
Olig2 (A) and Nkx2.1 (B) in the AEP (arrowheads) but not in
the diencephalon of megalin–/– embryos. (C) Expression
of Dlx2 is lost in the ventral telencephalon (arrowheads) and
significantly reduced in the ventral thalamus region of the diencephalon
(asterisks) of megalin–/– embryos as shown by
whole-mount ISH. (D) Immunohistology of coronal E10.5 forebrain sections
indicate lack of TuJ1 expression in the ventral (arrowheads), but not in the
dorsal, telencephalic region of megalin–/– compared
with control embryos. (E-H) Using whole-mount ISH, no differences can be seen
in the expression patterns of Ptch1 (ventral neural tube, including
tel-, di- and mesencephalon) (E), Hnf3b (ventral neural tube,
including di- and mesencephalon) (F), or Wnt1 (roof of di- and
mesencephalon, isthmus) (G), whereas expression of Pax6 extends from
the dorsal into the ventral neural tube of receptor-deficient embryos (H,
arrowhead). Also, most knockouts lack the Pax6 signal in the optic
vesicle. di, diencephalon; is, isthmus; me, mesencephalon; ov, optic vesicle;
te, telencephalon.
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