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Fig. 9. Megalin mediates binding and cellular catabolism of BMP4. (A) Surface
plasmon resonance (SPR) analysis of binding of 0.5 µmol/l BMP4 but not of
0.5 µmol/l BMP5 or WNT1-conditioned medium to purified megalin immobilized
on the sensor chip surface (see Materials and methods for details). As a
positive control, binding of 1 µmol/l receptor-associated protein (RAP) to
megalin was tested. (B) Megalin-expressing BN16 cells mediate uptake and
lysosomal degradation of 125I-BMP4. Degradation of BMP4 can be
blocked by chloroquine (200 µmol/l) or the receptor-antagonist RAP (100
µg/ml).
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