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Fig. 3. Re-expression of developmentally attenuated genes in human colorectal
cancers. (A) Normalization of cDNAs prepared from five of seven independent
pairs of tumors (T) and matched normal (N) colonic tissue by RT-PCR analysis
of ß-amyloid mRNA, which is known to be expressed to similar levels in
cancerous and normal human colon. Labels refer to serial dilution of cDNA
before PCR. (B) Expression (RT-PCR) analysis for the seven out of 27
transcripts that show increased expression in two or more of seven T-N pairs,
and illustrative results for two transcripts that show minimal change or
inconsistent differences between T and N. (C) Results were quantified by gel
densitometry, as shown here for one transcript, which encodes a 68 kDa
TGF-ß-induced protein. (D) Immunohistochemistry reveals the
representative oncofetal marker Trp53 to be expressed in tumor cells
and not in the surrounding stroma.
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