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Fig. 4. Crystallin expression and its regulation in Sip1-defective lenses.
(A-E) Normal lenses and (F-J) Sip1-defective lenses, subjected to
immunostaining (A-C,F-H) and in situ hybridization (D,E,I,J). (A,F) At E12.5,
-crystallins are expressed throughout the lens under either normal (A)
or Sip1-defective (F) conditions. ßA-crystallins are normally
expressed widely in nonepithelial domains through immature to mature lens
fibers (B), but in Sip1-defective lens, its expression was confined
to the innermost domain (G). 
-Crystallin expression marking mature lens
fiber cells in normal lens (C) is totally missing in Sip1-defective
lens (H), clearly indicating a defect in lens fiber maturation. Broken lines
indicate the boundaries of the bow region. However, two known key
transcription factor genes for -crystallin regulation, Sox1
(Kamachi et al., 1995;
Nishiguchi et al., 1998) and
Maf (Kawauchi et al.,
1999; Kim et al.,
1999; Ring et al.,
2000), are expressed in normal (D,E) and Sip1-defective
(I,J) lenses. Scale bars: 100 µm.
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