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Fig. 3. GPA-16 and GOA-1 become entirely dispensable for generation of pulling
forces following GPB-1 depletion. Average peak velocities±s.e.m. of
anterior (A) and posterior (P) spindle poles after spindle severing in
one-cell stage embryos of the indicated genotypes. Actual values are given in
Table S1 in the supplementary material. We found an analogous outcome when
examining the movements of centrosomes during centration/rotation prior to
mitosis: whereas centration/rotation is gradual, as in wild type, in embryos
compromised for either goa-1 or gpa-16 function, it is
abrupt and accompanied by back and forth movements in all genotypes in which
gbp-1 function is compromised (see Movies 1-7 in the supplementary
material). Values for wild type, goa-1(sa734) and
gpb-1(RNAi) are from Afshar et al.
(Afshar et al., 2004).
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