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Fig. 5. In segmentation mutants, neural crest migration is disrupted in the same
somites in which segmentation is disrupted. Whole-mount staining with a
crestin riboprobe of wild-type (A) fss (B), bea (C)
and smu mutants (D) at 21 hpf to reveal neural crest cells. (A) In
wild-type embryos, neural crest cells migrate in segmental streams along the
entire AP axis. (B) Neural crest migration is abnormal from the first somite
in fss mutants. (C) In bea mutants, neural crest migration
is disrupted posterior to somite 6. (D) In smu mutants there is no
clear segmental pattern of neural crest migration; in addition, neural crest
cells stall at the level of the dorsal aspect of the notochord. (E,F) EB165
antibody staining reveals that in smu mutants (F) somites are smaller
along the DV axis, but a significant amount of fast muscle is still present,
although there may be less than in wild types (E). Scale bar: 60 µm in A-D;
25 µm in E,F.
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