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Fig. 6. Fast muscle is not essential for neural crest migration on the medial
pathway. (A-D) Whole-mount staining of shh mRNA-injected embryo at 21
hpf. (A) crestin RNA expression (red) in neural crest cells. (B) F59
protein localization (green) in slow muscle. (C) Merged image. (D) DIC image.
In the absence of fast muscle, the somites do not adhere to one another,
creating a somite-free space in the intersomitic cleft. Neural crest migrates
in this region, presumably because neural crest cells are motile in the
absence of somites. However, neural crest also migrates in normal streams
medial to the middle of somites (arrows in C). (E,F) Cross-section of
shh mRNA-injected embryo. The somite on the left side of this embryo
has no fast muscle and excess slow muscle (all the muscle is slow, as
indicated by F59 staining in F); however, neural crest cells (crestin
riboprobe, purple in E) have migrated to the same extent as on the right side,
where fast muscle is present and slow muscle has migrated to the myotome
periphery, as in wild type. Scale bar: 20 µm.
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