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Fig. 2. AChR clustering precedes axon outgrowth. CaP axons (green) labeled with
znp-1 (B,C) or GFP (A,D-I) as in Fig.
1; AChRs labeled with BTX (red). (A-D) Segments 1-3. Neither axons
nor AChR clusters are present at 14 hpf (A), but clusters are present in front
of the advancing axon by 16-17 hpf (B,C). By 20 hpf, (D) most AChR clusters
are apposed by axonal varicosities, but some clusters are larger than
varicosities (arrowheads) and some axonal processes extend beyond clusters
(arrow). Asterisks in B-D indicate some of the AChR clusters that are not
innervated; they are quantified in Fig.
6F. (E-I) Whole mount of a 20 hpf fish (E), and higher-power
details of individual segments (F-I) showing the rostrocaudal developmental
sequence (see also Fig. 1A). In
caudal segments (F), AChR clusters have formed but no axon has yet extended.
In intermediate segments (G,H), clusters on adaxial cells not contacted by CaP
disperse (asterisks in E). In the most rostral segments (I), the CaP axon has
extended beyond the ventral-most AChR clusters. Scale bars: in A, 10 µm for
A-D; in E, 25 µm; in I, 10 µm for F-I.
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