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Fig. 5. Distinct modes of synapse formation on muscle pioneers and fast fibers.
Cross-sections through HB9:GFP fish at 20 hpf (A-D,F) or 28 hpf (E,G) were
stained with anti-myosin (F59) and BTX. (A-D) CaP extends to contact muscle
pioneers as the other adaxial fibers migrate radially. (E) CaP then extends
past the pioneers as fast, weakly F59-immunoreactive, fibers form. AChR
clusters on migrating adaxial cells are lost and new clusters form on fast
fibers as, or after, they are contacted by CaP. As in Figs
1 and
2, the rostrocaudal sequence of
development allows the visualization of many stages in a single fish. (F,G) En
face views at 20 hpf and 28 hpf to show that AChR clusters on muscle pioneers
are larger and more elongated than those on fast fibers. A'-G'
show F59 and BTX, but not axons, to highlight AChR cluster position and
morphology. Scale bar in E: 10 µm for all panels.
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