|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Responses of motor axons to Slit proteins in vitro. (A) Dissection of
tissues for use in collagen gel co-cultures (see Materials and methods).
(B,C,E) Trigeminal (r2+3) level explants co-cultured with cells transfected as
indicated. (D) Histogram showing quantitation (mean % pixels on facing side of
explant are on the y axis) of outgrowth from explants at r2-8 levels
in the presence of transfected cell clusters; n>30 for each
condition. Asterisks represent significant differences from the controls.
(F-M) Semi-quantitative data. A shift to negative explant net scores indicates
inhibition of outgrowth (see Materials and methods); inhibition was
significant when P<0.1 in all cases, and significant when
P<0.05 in G,H,I,K,M; n>25 for each condition. Scale
bar: 400 µm.
|
