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Fig. 3. Responsiveness of motoneuron subpopulations to Slit proteins in vitro.
(A,D,G) Immunostained trochlear axons from explants co-cultured with
transfected cell clusters. (B) Histogram showing mean angle difference
representing trochlear axon bundle chemorepulsion. Significant differences
from controls represented by asterisks (P<0.05); n>20
in each condition. (C,F,I) Spinal cord explants growing in the presence of
transfected cell clusters. (E,H) Histograms representing quantitation of mean
% of axons facing cluster (y-axis) for retrogradely labelled dorsally
projecting (BM/VM) axons (E) or ventrally projecting (SM) axons (H). Asterisks
indicate significant deviations from the control values (P<0.05);
n>10 for BM/VM labelled explants, n>25 for SM labelled
explants. (J-L) Explants placed in apposition to Slit1-transfected
cell clusters. (M-O) Explants facing mock-transfected control cell clusters.
(J,M) Ventrally projecting abducens axons. (K,N) Dorsally projecting
glossopharyngeal axons. (L,O) Dorsally projecting hypoglossal/cranial
accessory axons. Scale bars: 400 µm in I for A,C,D,F,G,I; 400 µm in O
for J-O.
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