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Fig. 7. The role of Slit-Robo signalling in motor axon pathfinding in the hindbrain. (A-D) Schematic diagrams of the vertebrate embryo hindbrain at stage 19 chick (A-C) or E11.5 mouse (D) is presented. Rhombomere levels are indicated (r) and the midbrain (MB)-hindbrain (HB) boundary. BM/VM neurons are shown in blue with corresponding exit points (blue ellipses) while SM neurons are shown in red. (A) Composite patterns of Slit and Robo expression (see key) in regions relevant to this study. (B) BM/VM axon pathway errors (thicker blue axons) in Slit1-electroporated sides of chick hindbrains (left-hand side) compared with non-electroporated or GFP-electroporated (right-hand side). Axon stalling and inappropriate, caudal projection of motor axons is shown. (C) Behaviour of BM/VM neurons electroporated with Robo dominant-negative GFP constructs (left-hand side; green axons), compared with myr-GFP electroporated axons (right-hand side; green axons). Stalling, misprojections and ectopic midline crossing is shown. (D) Behaviour of BM/VM axons in Robo2 mutant mice at E11.5 (left-hand side) compared with wild-type embryos (right-hand side). Ectopic midline crossing, longitudinal extension and looping are shown. Purple axons in D represent IEE axons which also show pathfinding errors. (E) Schematic of two rhombomeres with DiI labelling of the sensory ganglion outside the hindbrain, leading to labelling of motor axons and cell bodies within the hindbrain (see Materials and methods).

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