DEV02028 Supplementary Material

Files in this Data Supplement:

• Movie 1 - Movie 1. Animated time-lapse confocal images of a wild-type embryo expressing Histone-GFP after nuclei have migrated to the cortex. The movie shows a complete mitotic cycle with a well-synchronized mitotic wave throughout the embryo.

• Movie 2 - Movie 2. Animated time-lapse confocal images of a bubR1^Rev1 mutant embryo expressing Histone-GFP after nuclei have migrated to the cortex. The movie shows a complete mitotic cycle. Note that chromosomes never reach a stable metaphase organization and anaphase onset is not synchronized. Also, at the end of mitosis many nuclei show extensive DNA bridges, lagging chromosomes and irregular segregation.

• Movie 3 - Movie 3. Animated time-lapse confocal images of the polar body from a wild-type embryo expressing Histone-GFP. The movie shows that the polar body remains condensed throughout the recording period and there is no variation in the Histone-GFP signal level.

• Movie 4 - Movie 4. Animated time-lapse confocal images of the polar body of a bubR1^Rev1 mutant embryo expressing Histone-GFP. The movie shows that the polar body undergoes cycles of condensation and decondensation during the recording period (45 to 80 minutes) and there are cyclical changes in the histone-GFP signal level.