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Fig. 7. Live imaging and BrdU incorporation of the polar body of wild-type and bubR1^Rev1 embryos. (A,B) Time lapse imaging of polar bodies from fertilised (A) wild-type or (B) bubR1^Rev1 embryos. In wild-type embryos, polar bodies do not change in structure and Histone-GFP signal level remains constant. In bubR1^Rev1 embryos, polar bodies show cyclical changes in Histone-GFP signal intensity (see Movies 3 and 4 in the supplementary material). (C) Graphical representation of the relative Histone-GFP signal intensity/pixel in polar bodies of different embryos (wt, wild type; S1-4, bubR1^Rev1 embryos). Oscillations in Histone-GFP signal intensity represent differences in DNA condensation and decondensation. (D) Graphical representation of the dynamic range of the raw data from C. (E,F) BrdU incorporation into fertilised (E,E'') wild type or (F,F'') bubR1^Rev1 embryos. Arrows indicate polar bodies and arrowheads syncytial nuclei. BrdU incorporation occurs in the DNA of the bubR1^Rev1 polar body and in all the nuclei in the interior of the embryo, but not in the polar body of wild-type embryos. In all merged images, DNA is stained blue and BrdU is stained red. Insets in merged images show higher magnifications of the polar body. Scale bars: 10 µm in A,B; 100 µm E,F.

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