Temporally controlled targeted somatic mutagenesis in embryonic surface ectoderm and fetal epidermal keratinocytes unveils two distinct developmental functions of BRG1 in limb morphogenesis and skin barrier formation
Development Indra et al.
132: 4533
Supplemental Figure 1
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Fig. S1.
Morphological and functional analysis of E18.5 Brm–/–
fetuses. (A) Immunohistochemical detection of BRM on dorsal skin sections from
E18.5 control (CT) fetuses. Red staining corresponds to BRM antibody (a,b),
blue staining to DAPI (b). B, basal; SB, suprabasal; D, dermis. The dotted line
indicates the dermal-epidermal junction. Scale bar : 25 μm. (B) Gross morphology of E18.5 Brm+/+ and Brm–/– fetuses. (C) Barrier-dependent X-Gal
diffusion assay on E18.5 Brm+/+
and Brm–/– fetuses.
(D) Histological analysis of Toluidine Blue-stained semithin sections of dorsal
skin biopsies from E18.5 Brm+/+
and Brm–/– fetuses.
Epidermal cell layers are indicated: C, cornified; G, granular; S, spinous; B,
basal; D, dermis. Scale bar in a: 12 μm.
(E) Transmission electron microscopy of dorsal skin from E18.5 Brm+/+ and Brm–/– fetuses. SC, stratum corneum; SG,
stratum granulosum; SS, stratum spinosum. Scale bar: 4 μm.
