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Fig. 3. AprA is present in and is secreted by vegetative cells. (A) Cells growing
in shaking culture (vegetative cells), conditioned growth medium, and
conditioned starvation buffer were electrophoresed on an SDS-polyacrylamide
gel. A western blot of the gel was stained with affinity-purified anti-AprA
antibodies. `aprA-/a15::aprA' indicates
aprA-/actin15::aprA. (B) Growing cells were stained for
AprA by immunofluorescence. Scale bar: 20 µm.