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Fig. 1. Adrenal cortical cells are labelled by Sf1 in-situ hybridization
on a transverse section (dorsal, top) of an E13.5 wild-type embryo (A).
Sf1 expression is completely lacking in
Sf1–/– littermates (B). In-situ hybridization
for NF mRNA (blue) and TH immunofluorescence staining (red overlay) have been
performed on a consecutive section of the wild-type (C; E, higher
magnification) and the Sf1–/– embryo (D; F,
higher magnification). Note that in Sf1–/–
embryos TH+/NF– `adrenal' chromaffin cells
accumulate at a location where the adrenal gland develops in wild-type mice.
Scale bars: 100 µm. a, dorsal aorta; gn, gonadal anlage; sg, sympathetic
chain ganglion; srg, suprarenal ganglion.
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