|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Double mutant analysis of SDN-1 and HS-modifying enzymes. (A)
Circumferential guidance of DD commissures (L1 larvae), as shown by the
genetic interaction of sdn-1 nulls with HS-modifying enzyme mutants.
Data represent the number of commissures that reach the level of the DNC per
L1 larva. Error bars indicate s.e.m. (B,B') Analysis of DD commissures
in L1 larvae. (B') In hse-5; sdn-1 double-null mutants, most DD
commissures either fail to grow out dorsally or branch prematurely
(arrowhead). (C,C') VNC view of L1 larvae. (C') The VNC of
hse-5; sdn-1 double-null mutants is highly defasciculated
(arrowhead). (D,D') DD/VD commissures of L4 larvae. (D')
Circumferential guidance of DD/VD motoneurons is disrupted in hse-5;
sdn-1 double mutants (arrowheads). D and D' show confocal
z-stack images to visualize all misguided commissures in different
focal planes. (E) The PVQ crossover phenotype demonstrated by the genetic
interactions of sdn-1 null worms with HS-modifying enzyme mutants and
slt-1(eh15). Error bars indicate the standard error of proportion.
Asterisks denote statistical significance as follows:
***P<0.0001; n.s., not significant; n, number
of animals scored. Scale bars: 10 µm in B-C'; 25 µm in
D,D'.
|
