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Fig. 1. The downregulation of gcm precedes the induction of lz in
the anterior-most row of prohemocytes. (A,B) gcm (red) and
srp (green) transcription in stage 5 (A) or stage 6 (B) embryos. (C)
gcm transcript (red) and SRP protein (green) expression in stage 6
embryo. (D,E) lz-lacZ embryo processed to reveal gcm
transcript (red) and ß-gal protein (green) at stage 7 (D) and at stage 10
(E). (F-J) lacZ expression in lz-lacZ (F) or
lz-gal4/uas-gal4; uas-gal4/+; uas-gal4/uas-lacZ (G-J) embryos at
stage 7 (F,G, ventral views), stage 11 (H, side views) or stage 14 (I, side
views). (J) High magnification views of lacZ-expressing cells
localised in the crystal cell cluster (left panel) or far from the cluster
(right panels) in a stage 14 lz-gal4/uas-gal4; uas-gal4/+;
uas-gal4/uas-lacZ embryo. (K-M) Side views of gcm-lacZ embryos
processed to reveal DoxA3 mRNA (red) and ß-gal protein (green)
at stage 11 (K), 14 (L) or 15 (M). (N,L) Side views of DoxA3 mRNA
(red) and ß-gal protein (green) in srp-Gal4;uas-lacZ (N) or
lz-lacZ (O) embryos at stage 15. Contrary to lz-lacZ or
srp-lacZ, gcm-lacZ expression is progressively lost in the crystal
cells during embryogenesis: 26 out of the 28 DoxA3+ cells
are gcm-lacZ+ at stage 11 (K), against 9 out of 27 at
stage 14 (L) and 0 out of 27 at stage 15 (M). lz-lacZ codes for a
cytoplasmic ß-gal whereas gcm-lacZ and uas-lacZ code
for a nuclear ß-gal.
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