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Fig. 1. Potential mediators of Wnt signaling in the developing pancreas. (A,B) In
situ hybridization (purple) to E13.5 pancreatic primordia detects
co-expression of Pdx1 (A) and the ß-catenin-binding
transcription factor Tcf4 (B), while the related factor Lef1
is not expressed (data not shown). (C-D') To confirm the specificity of
pan-specific (C,D) and dephospho-specific (C',D')
anti-ß-catenin monoclonal antibodies, we immunostained (brown)
near-adjacent sections of embryonic spinal cord and neonatal (P0) intestine.
As expected, anti-dephospho-ß-catenin recognizes regions where Wnt
signaling is known to be active, such as the ventricular zone of the spinal
cord (arrow) and the intestinal crypts (arrowhead). (E-I')
Pan-ß-catenin (E-I) and dephospho-ß-catenin (E'-I')
staining was similarly performed on various stages of developing pancreas.
Arrowheads and arrows indicate morphologically recognizable islet and acinar
tissue, respectively. Dephospho-ß-catenin is specifically detected in the
early pancreatic epithelium, and declines as the organ matures. me, pancreatic
mesenchyme; ep, pancreatic epithelium.
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