DEV02062 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. fil yab3 mutant fruit. (A) Mature wild-type fruit (stage 19) with valves detaching from fruit. (B) Mature fil yab3 fruit. Valves do not readily detach. (C) At the base of fil yab3 fruit, some valve detachment can occur. (D) Cross section of a fil yab3 fruit taken from the apical region and stained with Saffranin O and Alcian Blue. The lignified layer and separation layer are missing. (E) Cross-section of a fil yab3 fruit taken from the basal region of the fruit and stained with Saffranin O and Alcian Blue. The brackets mark regions of the fruit in which separation and lignified layer tissues have expanded from the valve margin into the valves. The arrowhead marks a stripe of ectopic valve margin tissue in the center of the valve. r, replum; v, valve. Scale bars: 1 mm (A,B), 0.5 mm (C), 50 mm (D), 100 mm (E).

• Supplemental Figure 2 -

  Fig. S2. Expression of genes important for fruit patterning during early gynoecium development. FIL, JAG and FUL expression detected by in situ hybridization in wild-type gynoecia. SHP2::GUS reporter used to assay SHP2 expression. Floral development stages are indicated above. r, replum; v, valve. Scale bars: 50 mm (applies to panels at the same floral stage).

• Supplemental Figure 3 -

  Fig. S3. JAG promotes leaf blade growth redundantly with FIL and YAB3. (A) A fil yab3 mutant develops some blade tissue on the leaves. (B) In jag fil yab3 mutants, leaf blade development is strongly suppressed. Scale bar: 3 mm (A,B).

• Supplemental Figure 4 -

  Fig. S4. FUL GUS activity in jag and fil jag fruit (~stage 16). (A) In wild-type fruit, reporter activity is limited to the valves. (B) A small cleft of FUL-negative tissue develops near the apical shoulder of jag valves. (C) This cleft of FUL-negative tissue expands further in the jag fil mutant fruit.

• Supplemental Figure 5 -

  Fig. S5. The effect of the jag-5D mutation on JAG and FIL expression. (A) Expression of JAG detected by in situ hybridization in a stage 8 gynoecium. Ectopic JAG expression is expanded into the replum region (black arrowhead). (B) Expression of FIL detected by in situ hybridization in a stage 8 gynoecium. FIL expression in jag-5D mutants is similar to wild type. r, replum. Scale bar: 50 m (A,B).

• Supplemental Figure 6 -

  Fig. S6. AG expression in fil yab3 mutants is similar to wild type. The KB9 GUS reporter was used to monitor AG expression. (A) Stage 3 wild-type flower. Reporter activity is present between the sepal primordia. (B) Stage 11 wild-type gynoecium. Reporter activity is maintained in the septum and ovules. (C) Late stage 3 fil yab3 flower. (D) Stage 11 fil yab3 gynoecium. Scale bars: 50 mm.