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Fig. 1. Wild-type fruit development. (A) Bright-field image of a stage 17 fruit. (B,C) SEM of the apical region (B) and basal region (C) of a fruit. (D) Diagram of a cross section of a fruit with the various tissues indicated. (E) Safranin O- and Alcian Blue-stained cross section near the valve margin. The separation layer stains light blue and the lignified layer stains pink. (F,G) Phloroglucinol-stained cross sections of the valve margin (F) and valve (G). This stain is lignin specific and marks the lignified layer of the valve margin, the enb layer and cells of the vascular bundle. v, valve; r, replum; vm, valve margin; st, style; gy, gynophore; SL, separation layer; LL, lignified layer; vb, vascular bundle. Scale bars: 1 mm (A), 200 µm (B,C), 50 µm (E-G).

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