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Fig. 3. FIL and YAB3 are necessary for proper SHP2 and
FUL expression. GUS expression driven by the SHP2
promoter (A-E) or the ful-1 enhancer trap line (F-I). All plants
stained for FUL GUS activity are heterozygous for ful-1. (A)
In wild-type fruit, the SHP2::GUS reporter is active in the valve
margins throughout the fruit at stage 14. (C) In cross section, reporter
activity is visible in the ovules as well as the valve margins. (B,D,E) In
fil yab3 fruit, SHP2 reporter activity is lost in the apical
region of the fruit (B –,D) while ectopic reporter activity is present
in the valves of the basal portion (B ++,E). (F,G) FUL GUS staining
of a stage 12 wild-type gynoecium showing expression in the two valves as well
as in the vasculature of the style (F) and replum (G). (H,I) In the fil
yab3 gynoecium, reporter activity is absent from the valves but remains
in the vasculature. Note the presence of a supernumerary carpel in the fil
yab3 gynoecium (I). (J-N) Lignin staining of valve cells in mature fruit
(stage 
17). (J) 35S::FUL valves develop a lignified enb
layer similar to wild type. (K) 35S::FUL is able to suppress most of
the ectopic lignification that occurs at the base of fil yab3 mutants
and is able to rescue enb layer lignification in the apical region
(L). Note some ectopic lignification is still present in the basal region. (M)
In ful mutants, valve mesophyll cells become ectopically lignified as
a result of the expanded expression of valve margin identity genes. (N) In the
apical region of ful fil yab3 fruits, however, all cells of the
valves are unlignified including the enb layer, suggesting that the
valve margin identity genes are not active. v, valve; vm, valve margin; r,
replum; ov, ovule; st, style; vb, vascular bundle; gy, gynophore; EL, ectopic
lignification. Scale bar: 0.5 mm (A), 1 mm (B), 100 µm (C,D,E), 200 µm
(F,H), 50 µm (G,I-N).
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