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Fig. 4. JAG acts redundantly with FIL and YAB3 to control valve margin patterning. (A) Expression of JAG detected by in situ hybridization in a stage 8 wild-type gynoecium. JAG is expressed in the valves and presumptive valve margin region and is expressed in all cell layers. (B) SEM of the apical region of a jag fruit. The shoulders of the valves are sloped downwards in jag. (C) SEM of a jag fil fruit. The style/ovary (white arrowhead) and gynophore/ovary (black arrowhead) boundaries have shifted, with the style and gynophore becoming enlarged. (D) jag fil fruit develop a stripe of valve margin tissue that runs down the middle of the valves. (E) In cross sections stained with Safranin O and Alcian Blue, separation layer and lignified layer cells are present overlying the vascular bundle. (F) SEM of jag fil yab3^+/– fruit showing further expansion of style and gynophore into the ovary region. (G) SEM of jag fil yab3^+/– ER fruit. The erecta mutation suppresses the growth of the style as it does in ful mutants (Ferrándiz et al., 2000a). (H) Close-up SEM of a jag fil yab3^+/– replum. The normally narrow ridge of tissue has expanded in width and twists throughout the length of the fruit similar to the replum of ful mutants (J). (I) SEM of a patch of small cells that form in the valves of jag fil yab3^+/– fruit. These cells appear very similar to the ectopic valve margin cells that replace the valves in ful mutants (K). (L) SEM of a jag fil yab3 mutant flower. All the floral organs have patterning defects. Sepals, petals and stamens are replaced by filamentous organs with no apparent floral characteristics. (M) SEM of a fertilized jag fil yab3 fruit. No valve margin development is present. (N) Epidermal cells of jag fil yab3 valves are elongated with stomata interspersed (white arrowheads), similar to wild type. Occasionally, however, cells with an irregular shape can be seen. (O,P) Cross sections at the base of a jag fil yab3 fruit stained with Safranin O and Alcian Blue. All cell types necessary for dehiscence, including the lignified layer, separation layer and enb layer are absent. Some enb lignification may be present internally to the vascular bundle. r, replum; v, valve; vm, valve margin; LL, lignified layer; SL, separation layer; vb, vascular bundle. Scale bar: 50 µm (A,E,I,K,N,O,P), 200 µm (B,L), 1 mm (C,G,M), 100 µm (D,H,J), 500 µm (F).

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