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Fig. 5. JAG promotes FUL and SHP expression redundantly
with FIL and YAB3. (A-D) FUL GUS reporter activity
in the gynoecium using the ful-1 enhancer trap line. (E-N)
SHP2::GUS reporter activity in stage 15-16 fruit (E-J) and stage 12
gynoecia (K-N). (A,B) Whole mount (A) and cross section (B) of FUL
GUS staining in a stage 12 jag fil yab3+/–
gynoecium showing reporter activity restricted to small islands corresponding
to the valves. (C,D) Whole mount (C) and cross section (D) of FUL GUS
staining in a stage 12 jag fil yab3 gynoecium. Reporter activity is
completely absent from the valves. (E,F) Frontal (E) and side (F) views of
SHP2::GUS expression in wild type. (G) In jag mutant fruit,
a small wedge of ectopic reporter activity is detected in the apical region of
the valves (black arrowhead). (H) In fil mutant fruit, a stripe of
ectopic reporter activity can be seen extending through the center of the
valve, overlying the vasculature. (I) In fil yab3+/–
mutant fruit, the intensity of reporter activity in the valves is stronger
than in fil single mutants. (J) In jag fil mutant fruit,
strong reporter activity is present in a stripe in the valves with weaker
staining elsewhere. (K,L) Cross section of a jag fil
yab3+/– gynoecium showing strong ectopic reporter
activity in the valves. Using dark-field microscopy, strong GUS staining
appears purple, whereas weak staining appears pink/orange. (L) The same
section as in K viewed under bright-field microscopy. (M,N) Cross section of a
jag fil yab3 gynoecium showing weak reporter activity in the valves
(M). Using bright-field microscopy (N) to image the section in M, the loss of
SHP2::GUS reporter activity in the valves and valve margins is even
more apparent, compared to the strong GUS staining seen in the valve regions
of jag fil yab3+/– gynoecia (L). v, valve; r,
replum; ov, ovule and st, style. Scale bars: 200 µm (A,C); 100 µm (B,D);
0.5 mm (E-J); 100 µm (K-N).
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