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Fig. 4. Nuclear pMAPK following Spitz activation in S2 cells. S2 cells were stained
for Egfr antigen, pMAPK antigen and DNA as indicated. Conditions are as in
Fig. 2A and
Fig. 3, see text. Transfected
DNA and temperature are indicated on the left. Transfected cells express
elevated Egfr antigen (arrowheads), untransfected control cells do not. Note
that cells transfected with Egfrtsla, at 30°C, do not
express pMAPK over background levels. Arrows indicate increased levels of
pMAPK antigen. Scale bar: 5 µm.
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