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Fig. 2. Migration of GN11 cells is inhibited by reelin-rich olfactory bulb explants
in three-dimensional collagen matrix. Olfactory bulb explants (OB) from E18
rats were cultured with GN11-EGFP cells in collagen gels. (A) GN11-EGFP cells
(green) migrated away from the OB (red, axonal staining). (B) The zone
surrounding the cell aggregates was divided into quadrants, thus defining a
proximal (P) and a distal (D) quadrant in relation to the OB. (C) Migrating
cells were counted in 24 co-cultures and expressed as a percentage of cells
± s.e.m. in each of the two quadrants out of the total number of cells
in both quadrants. We found that 66.54±1.40% of cells migrated into D
and 33.46±1.40% into P. Scale bar: 250 µm. (D,E) Addition of CR-50
antibody resulted in roughly equal proportions of GN11-EGFP cells in P
(53.53±2.60%) and D (46.47±2.60%; n=12). Scale bars:
250 µm.
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