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Fig. 1. Activity of the core element of the Foxa2 node/notochord enhancer
in transgenic mouse embryos. (A) Schematic representation of the mouse
Foxa2 enhancer. The node/notochord enhancer is located upstream of
the transcription initiation site (yellow square). CS3 (red circle) is
essential for enhancer activity. A 27-bp DNA fragment straddling CS3 is the
core element (CE) of the enhancer. (B-J) Distribution of ß-galactosidase
activity in transgenic mouse embryos that express LacZ under the
control of eight copies of the CE. Whole-mount staining of LS (B), EB (C), LHF
(D,E), E8.5 (G) and E9.0 (H) stage embryos. Sagittal section of an LHF embryo
crossing through the node (F), and cross-sections of an E8.5 embryo (I,F).
Approximate positions of sections shown in panels I and F are indicated in
panel G by i and j, respectively. ame, axial mesoendoderm; ec, ectoderm; en,
endoderm; h, heart; hf, headfold; hm, head mesenchyme; m, mesoderm; mb,
midbrain; n, node; nc, notochord; nf, neural fold; np, neural plate; ov, otic
vesicle; pm, presomitic mesoderm; pp, prechordal plate; ps, primitive streak;
s, somite; tb, tailbud.
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