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Fig. 3. Hr-POPK-1 acts upstream of macho-1. (A) Normal uninjected larva. (A') Larva injected with POPK-1 mRNA. Head with pigment cells and kinked tail are recognizable. (B,B') Expression of AChE in uninjected embryo (B), and in embryo injected with POPK-1 mRNA (B'). Cleavage was arrested at the 110-cell stage. No ectopic muscle formed. (C-E) Expression of AChE in uninjected tailbud embryos (C), in embryos injected with macho-1 MO (D) and in embryos co-injected with macho-1 MO and POPK-1 mRNA (E). Loss of muscle cell was not rescued by co-injection of the mRNA. (F) Expression of AChE in embryos injected with control MO (left), in embryos co-injected with POPK-1 MO and macho-1 mRNA (center) (excess amounts of muscle cells are formed), and in embryos injected with POPK-1 MO (right) (no muscle is formed). Scale bars: 100 µm.





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