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Fig. 7. Type I postplasmic/PEM RNA localization on isolated cortices of
eggs and 8-cell embryos. cER was stained with DiIC18 (3) and emits
red fluorescence. Particle with intense red fluorescence in left panels are
micelles of DiIC18 (3). In-situ hybridization signals of
Hr-POPK-1, Hr-ZF1 and Hr-Wnt-5 are green. (A-D) Fluorescent
images of the cortices isolated from unfertilized eggs. (A) cER (left) and
Hr-POPK-1 mRNA (middle). The right panel shows a merged image. (B)
cER (left), Hr-ZF1 (middle) and a merged image (right). (C) cER
(left), Hr-Wnt-5 (middle) and a merged image (right). (D) cER (left),
macho-1 (middle) and a merged image (right). White arrowheads in A-C
indicate small granules with bright green fluorescence. (E-G) Confocal images
of the CAB domain in the posterior cortices isolated from 8-cell stage embryo.
(E) Hr-POPK-1 mRNA. (F) Hr-ZF1 mRNA. (G) Hr-Wnt-5
mRNA. Scale bars: 5 µm.
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