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Fig. 8. Confocal images of the cER/mRNA domain in the posterior cortices in
uninjected embryo and embryo injected with POPK-1 MO. cER was stained
with DiIC18 (3) and emits red fluorescence. In-situ hybridization
signal of Hr-PEM1 is green. (A) cER (left) and Hr-PEM1
(middle) in cortex that was isolated from an uninjected embryo at 8-cell
stage. The right panel shows a merged image. (B) cER-rich domain and
Hr-PEM1-rich domain overlap in cortices isolated from embryo injected
with POPK-1 MO. White arrowheads indicate the cER-rich region.
cER/mRNA domain is smaller and rounded in embryos injected with
POPK-1 MO. (C-E) Live cortices isolated from 2-, 4-, and 8-cell
embryos, respectively. (F) cER and Hr-PEM1 mRNA in posterior cortices
isolated from 4-cell stage embryo. Hr-PEM1-rich domain overlaps with
the cER-rich domain, as shown by the merged image (right). Scale bars: 10
µm.
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