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Fig. 1. Subcellular localization of the WER-GFP. (A) Schematic diagrams of PWER:GFP and PWER:WER-GFP gene constructs. The stop codon of the WER was deleted for the translational fusion with the GFP. (B) Complementation of the wer mutation with PWER:WER-GFP. The wer-1 mutant was transformed with PWER:WER-GFP. wt, wild type. (C) WER-GFP accumulation in the Arabidopsis root epidermal cells. The wer-1 mutant transformed with PWER:WER-GFP and the wild-type plant transformed with PWER:GFP were grown on agarose-solidified media. Fluorescence was observed in the 4-day-old seedlings using confocal microscopy.





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