|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Effect of the WER protein on CPC expression in
Arabidopsis. (A,C) Schematic diagrams of the reporter genes and the
effectors used in this transient expression assay (A) and stable transgenic
study (C). For reporter constructs, several versions of CPC promoters
that are 700 bp long (from the translational start site) were inserted
upstream of the luciferase gene (A) or ß-glucuronidase gene (C). Each
mutant reporter contains mutations at WBSI or WBSII, or both. For the effector
construct, the genomic DNA fragment of WER-coding region was inserted
downstream of the 35S promoter (A). (B) Importance of WBSI and WBSII (refer to
A) in CPC expression in the Arabidopsis protoplast transient
expression assay. Protoplasts were transfected with UBQ10-GUS as an internal
control, each of the reporters (wild type, M1, M2 or M3) and an effector
(WER). (D) Importance of WBSI and WBSII (refer to C) in the proper expression
of CPC in the Arabidopsis root epidermis. The seedlings were
stained for 24 hours.
|
