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Fig. 2. Mitotic progression is affected in the VNC of borr mutant embryos.
(A) Counts of mitotic cells, as judged by P-H3 staining and chromatin
morphology (see also Fig.
4B,D), in the VNC of wild-type or borr mutant embryos at
stage 12 (n=6 or 7, respectively), and at stage 14 (n=12 or
10, respectively). (B,C) Relative frequencies of the four main mitotic stages
(see D), expressed in percentages at stage 12 (B) and stage 14 (C). Asterisks
in A-C indicate statistical significance (**P<0.0005) of the
observed differences. (D) Examples of mitotic cells in which chromatin has
been visualised by P-H3 staining, selected to illustrate the four main mitotic
stages of wild-type and borr mutant VNC cells. There is abnormal
chromatin segregation in the mutant anaphase.