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Fig. 3. Subcellular localisation and function of Borr in cultured
Drosophila cells. (A-G) Mitotic DmD8 cells transiently expressing
GFP-Borr, stained with Hoechst dye and antibodies against ß-tubulin,
Aurora B or Incenp, as indicated. (H-M) Kc167 cells, (H) mock-treated or (I-M)
treated with borr dsRNA. Staining is with Hoechst dye (blue) and
phalloidin (red), and with antibody against ß-tubulin (green). Arrows
indicate DNA fragments in the cytoplasm of a multi-nucleate Borr-depleted cell
(M).