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Fig. 4. Reduced P-H3 levels in VNC cells of borr mutant embryos. (A-D)
Ventral views of stage 13 (A,B) wild-type or (C,D) borr mutant
embryos, stained with Hoechst dye and antibody against P-H3; projections of
z-stacks of confocal sections with identical settings are shown,
revealing lower numbers of mitotic cells in the mutant and reduced P-H3 levels
(see also Fig. 2A,D). (E-J)
Individual mitotic VNC nuclei prior to anaphase from (E-G) wild-type or (H-J)
borr mutant stage 13 embryos; arrow in I indicates a mutant nucleus
with a degree of chromatin condensation similar to that in wild type (F).
There are reduced levels of P-H3 staining in both mutant nuclei (J, compare
with G).
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