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Fig. 6. Delayed apoptosis of large borr mutant cells. (A) Third larval
instar wing disc, bearing early-induced borr mutant clones (marked by
absence of GFP, indicated by blue arrow) that are invariably small; red arrow
indicates large twin spot (showing strong GFP fluorescence), which lacks an
associated borr mutant clone. (B) Third instar larval
Minute/+ wing disc, bearing early-induced borr mutant clones
(that are Minute+ and thus have a proliferative
advantage), stained with Hoechst dye, showing numerous large borr
mutant cells that have apparently survived multiple abnormal mitoses. (C,D)
Wing disc as in A, showing DNA (blue), ß-tubulin (red, to mark cellular
outlines) and Aurora B staining (green, to mark normal mitotic cells within
the apical plane, indicated by arrowheads). Dying mutant cells are extruded
basally (bottom). Both images are optical 3D reconstructions through the pouch
regions of the disc along the y (C) or x (D) axis, generated
from a z-stack of 0.25 µm confocal sections. (E) Wing disc as in
A, stained with Hoechst dye and antibody against active caspase. Arrows in C-E
indicate large borr mutant cells that are healthy (grey), apoptotic
(white) or intermediate (red).
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