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Fig. 10. Sxl induces disc growth which can be non autonomous. (A) Wild-type disc; (B-C) cross sections of wild-type discs (B) and male (C) and female (D) discs expressing Sxl in the dorsal compartment stained for Ci (red) and Wg (green). Broken line in A indicates the position of the cross section. Arrowheads mark the Wg stripe at the D/V boundary of the wing pouch and at the dorsal end. Arrows indicate the folds in the dorsal wing pouch caused by disc overgrowth. (C) In the male disc, the fold near the dorsoventral border is over the ventral half of the disc and an additional fold is over the dorsal half. (D) The female disc cross-section is at a slight angle to show the folds and thickening in the dorsal half. Ventral is towards the left. Confocal image stacks taken with a 40x objective (female with zoom at 0.9). (E,F) Male (E) and female (F) discs expressing Sxl in only the dpp zone of anterior compartment stained for Ci (red) and Wg (green). Arrows indicate more severe curve of Wg stripe caused by bulge from growth. Compare with the gentle curve of Wg stripe in the wing pouch of the wild-type disc in A, which normally is relatively flat. Arrowheads in E,F indicate folds in wing pouch in posterior compartment caused by increased growth.





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