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Fig. 8. Sxl-null clones in females show reduced Hh signaling. (A-C) Small Sxl-null clone near AP border (arrowhead) identified by loss of GFP signal (A), shows a reduction in Ci levels (B); (C) merged image. (D-G) Larger Sxl-null clone in notum region of wing disc (arrowhead) identified by loss of GFP (D) and Sxl signal (E), showing a reduction in Ci levels (F); (G) merge of D-F. Sxl and Ci are primarily cytoplasmic as no LMB was used. (H-K) Sxl-null clones (arrowheads), identified by loss of GFP (H), stained for ß-gal (I) and Sal (J); (K) merged image. ß-gal, which reflects dpp transcription, and Sal levels show a slight reduction. Arrow indicates slightly higher ß-gal in non-clone region. The few nuclei with slightly brighter ß-gal signal near bottom clone are wild type for Sxl (small arrowhead in (I)). Scale bars: in G, 20 µm for D-G; in K, 20 µm. Anterior towards the left, ventral is towards the top. Confocal images throughout.





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